Rhodiola Rosea Extract in Dermocosmetic

Rhodiola Rosea Extract In Dermocosmetic

Rhodiola Rosea L. is a herb that grows in the cold, (ASL.1,000m~5,000m) mountainous region of Europe and Asia. Its root when cut gives off the aroma of a rose -and that’s why it is named Rhodiola ROSEA.

Due to the harsh growth environment, such as lack of oxygen, low temperature & drying, large temperature difference between day and night, it has strong vitality and adaptability. Well-known for its excellent effects in eliminating fatigue, anti-oxidation, anti-depression, anti-microwave radiation and anti-aging, it is also named "the plateau ginseng" in China. Its root contains more than 140 active ingredients, the two most potent of which are rosavin and salidroside. Rhodiola rosea extract is not only used for dietary supplement ingredient, but also for a wide range of applications in cosmetics. 

As a Natural Cosmetics Raw Material,Rhodiola Rosea Extract has the Following Advantages:

·Enhances skin's resistance to ionizing radiation and electromagnetic radiation effectively.   

·Inhibits tyrosinase activity, remarkable whitening function.

·Superior SOD activity. Superoxide dismutase can remove Free radicals and repair damaged skin.

Based on those advantages above, rhodiola rosea extract is widely used in the following Dermocosmetic

1.Anti-wrinkle, Anti-aging cosmetics

2.Anti-radiation cosmetics 

3.Sunblock & whitening cosmetics

4.Protect skin against external environmental stimuli cosmetics

5.Long-lasting moisturizer cosmetics

Biochemical and Cytological Efficacy Evaluation of Rhodiola Rosea Extract Applied to Dermocosmetic

Scholars from the College of Life Sciences of Sichuan University used biochemistry and cytology to explore the effects of Rhodiola rosea tyrosinase inhibition, DPPH free radical scavenging, melanin inhibition, and anti-aging. For the different effects, they did the following experiments:

1.DPPH Free Radical Scavenging Test

Natural plant extracts have unique molecular structure and cell biological functions, including components that inhibit tyrosinase activity, such as chamomile flower, which are characterized by safe, non-toxic side effects. It is increasingly used in Chinese herbal medicine raw materials.

Prepare PBS test drugs with Rhodiola extract liquid fractions of 0.1%, 0.5%, 1%, and 5%, take 10ul respectively, add 0.2mol/L absolute ethanol solution DPPH solution 90ul, set kojic acid as a control experiment In the group, the effect of Rhodiola rosea extract on inhibiting tyrosinase under different test volume fractions was tested. The experimental results are shown as follow.     

The experimental results showed that Rhodiola rosea extract had significant tyrosinase inhibition. When the liquid integral number of Rhodiola rosea was 0.1%, 0.5%, 1% and 5%, the tyrosinase inhibition rate was 15%. 48%, 57% and 72% showed a certain dose-effect relationship within the range of the tested volume fraction, showing a good tyrosinase inhibition. The tyrosinase inhibitory effect of Rhodiola rosea extract having a volume fraction of 5% was equivalent to the level of kojic acid compared to the positive control kojic acid.

As a high-quality natural plant and Chinese herbal medicine resource, Rhodiola rosea has a significant inhibitory effect on mushroom tyrosine in vitro, which can reduce the formation of melanin in the skin and whiten the complexion.

2. The Effect of Rhodiola Rosea Extract on DPPH Free Radical Scavenging Activity

It has been reported that some antioxidants such as green tea can effectively inhibit oxidative stress and prevent disease and aging. ROS from oxidative stress are affected by environmental factors such as UV radiation, smoking, pollution, ozone and certain drugs, chemicals or pesticides.

In this experiment, the antioxidant activity of compounds or plant extracts was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Rhodiola rosea extract was treated differently. The scavenging activity of DPPH free radicals under the test volume fraction is as follows：

The DPPH free radical scavenging rate characterizes the antioxidant activity of the test sample. In general, the higher the DPPH free radical scavenging rate, the stronger the antioxidant activity. Rhodiola rosea extract has strong anti-oxidation and scavenging harmful free radicals. When the volume fraction is 0.125% and 0.25%, the DPPH free radical scavenging rate of Rhodiola rosea extract reaches 71% and 86%. A certain dose-effect relationship is presented within the range of test volume fractions. This indicates that Rhodiola rosea extract has better anti-oxidation and oxidative stress damage protection, and achieves the effects of preventing diseases and anti-aging. 

3.Cytotoxicity of Rhodiola Rosea Extract   

The cytotoxic effects of Rhodiola rosea extract (extract and extract powder) were evaluated by HaCat cells and 1079SK cells. The results are shown in Figure 3.

The results showed that when the liquid integral number of Rhodiola rosea was 4% and 2%, it had a very significant cytotoxic effect on HaCat cells, and the cell viability was significantly reduced. The morphology of the cells was observed by inverted microscope, and the death of HaCat cells was obvious. When the mass concentration of the powder reached 1 g/L, it had no cytotoxic effect on 1079SK cells. Therefore, the test concentration of the Rhodiola extract applied to the cytological efficacy evaluation extract and the extracted powder of the cosmetic is selected to be equal to or lower than 1% and 1 g/L.    

4.Inhibition of Melanin Melanin by Rhodiola Rosea Extract

The results are shown in Fig. 4. The effect of Rhodiola rosea extract on melanin production in mouse B16 melanoma cells was observed under an inverted microscope. Rhodiola rosea extract significantly reduced IBMX-induced B16 melanoma in mice at 1% tested concentration. Cell melanin production, while its melanin content is significantly lower than the IBMX induced melanin content.

The results are shown in Fig. 5. Rhodiola rosea extract can significantly reduce the production of melanin in human G361 melanoma cells induced by IBMX at a concentration of 0.5 g/L. The inverted microscope and the melanin content test indicate that the Rhodiola The extract of the sky has a good effect of inhibiting melanin production, and the melanin content secreted by the cells is remarkably reduced.

Chinese herbal extracts have been reported to inhibit the production of melanin by melanocytes induced by IBMX, such as scorpion . The test showed that Rhodiola rosea extract had better tyrosinase inhibition, and the melanoma cell model test also showed that it had better inhibition of melanin production of melanin, indicating that red Sedum extract has the effect of inhibiting melanin and whitening in vitro by tyrosinase and cell water, and has high application value.

5 Anti-aging Effect of Rhodiola Rosea Extract on Fibroblasts

In order to study the anti-aging effect of Rhodiola rosea extract, its effect on the proliferation of fibroblast 1079SK cells was evaluated in vitro. 1079SK fibroblasts were treated with Rhodiola rosea extract with a volume fraction of 0.05%. After cultured for 48 h in a cell culture incubator, the cell density was measured by MTT assay and the cell proliferation rate was evaluated. The results showed that, as shown in Fig. 6, Rhodiola rosea extract can promote the proliferation of 1079SK fibroblasts, and the number of cells increased by 18.93%, which significantly promoted the proliferation of fibroblasts and enhanced the ability of cell regeneration to achieve anti-aging effects. 

At the same time, the protective effect of Rhodiola rosea skin 1079SK fibroblasts on UVA photodamage was evaluated in vitro, and the effect of inhibiting photoaging and anti-aging on the skin was tested.

After the skin 1079SK fibroblasts were irradiated with 20 J/cm2UVA, the cell survival rate decreased, and the cell survival rate of the 1079SK fibroblasts treated with the volume fraction of 1% Rhodiola rosea was significantly restored after UVA irradiation, reducing UVA. Photodamage effect on fibroblasts. This indicates that Rhodiola extract can effectively repair fibroblasts in the dermis of the skin, reduce photo damage caused by UVA, resist UVA photoaging damage to the dermis, protect dermal cells, delay skin cell damage, and achieve anti-aging effects.

6.Effects of Rhodiola Extract on ATP Content in HaCat Cells

Adenosine triphosphate (ATP) is formed in mitochondrial nucleoside triphosphates and is a high-energy small molecule compound in cells. The ATP content of HaCat cells was measured by the ATP Colorimetric/Fluorometric Assay Kit. The results showed that after treatment of HaCat cells with different concentrations of Rhodiola rosea extract for 24 h, the concentration of Rhodiola rosea extract was 0.05 g/L, the ATP of HaCat cells was significantly increased, and ATP increased by 19%.

ATP is a direct source of energy for various life activities in living organisms. The increase of ATP content in cells can promote the repair and regeneration of cells. The level of ATP in cells directly reflects the vitality of cells . The effects of Rhodiola rosea L. extract on ATP content in HaCat cells were studied. The results showed that Rhodiola rosea extract could increase the ATP content of HaCat cells and promote cell metabolism and activity.

The research investigated the activity of Rhodiola rosea tyrosinase inhibition, DPPH free radical scavenging, melanocyte melanin inhibition, anti-aging, ATP promotion. The results showed that Rhodiola rosea extract and Rhodiola rosea extract could effectively inhibit mushroom tyrosinase activity, inhibit melanoma production of melanoma cells, improve skin color and whitening effect. Meanwhile, Rhodiola rosea extract can Free radical scavenging, reduce oxidative stress damage, promote fibroblast proliferation, repair fibroblast UVA irradiation damage, prevent skin sunburn, and achieve deep anti-aging effect; Moreover, Rhodiola rosea extract can significantly increase HaCat cells ATP production increases cell activity and ATP energy turnover. Therefore, Rhodiola has broad prospects in the development and application of functional products such as whitening and anti-aging for daily use cosmetics.